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Recruitment of substrate-specificity properties from one enzyme into a related one by protein engineering.

The Bacillus licheniformis and Bacillus amyloliquefaciens subtilisins differ by 31% in protein sequence and by factors of greater than 60 in catalytic efficiency, kcat/Km, toward various substrates. Despite large differences in sequence and substrate specificity for these serine proteases, only two...

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Dettagli Bibliografici
Autori principali: Wells, J A, Cunningham, B C, Graycar, T P, Estell, D A
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1987
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC298815/
https://ncbi.nlm.nih.gov/pubmed/3299378
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