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Cloning and biochemical characterization of a novel lipolytic gene from activated sludge metagenome, and its gene product
In this study, a putative esterase, designated EstMY, was isolated from an activated sludge metagenomic library. The lipolytic gene was subcloned and expressed in Escherichia coli BL21 using the pET expression system. The gene estMY contained a 1,083 bp open reading frame (ORF) encoding a polypeptid...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2987919/ https://ncbi.nlm.nih.gov/pubmed/21054894 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-9-83 |
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