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Transcriptional activity of the human thymidine kinase gene determined by a method using the polymerase chain reaction and an intron-specific probe.

We have used the technique of reverse transcription coupled to the polymerase chain reaction to detect mRNA precursors [heterogeneous nuclear RNA (hnRNA)] transcribed from the thymidine kinase (TK) gene of human diploid fibroblasts. With this method, the amplification products of both hnRNA (contain...

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Dettagli Bibliografici
Autori principali: Lipson, K E, Baserga, R
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1989
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC298584/
https://ncbi.nlm.nih.gov/pubmed/2481317
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