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Transformation of Tetrahymena thermophila with a mutated circular ribosomal DNA plasmid vector.

A circular plasmid containing a complete Tetrahymena thermophila rRNA gene (rDNA), with a tandem repeat of a 1.9-kilobase-pair segment encompassing the replication origin and the rRNA promoter, and a polylinker in the 3' nontranscribed spacer, was used to transform T. thermophila by microinject...

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Bibliographic Details
Main Authors: Yu, G L, Blackburn, E H
Format: Artigo
Language:Inglês
Published: 1989
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC298307/
https://ncbi.nlm.nih.gov/pubmed/2813408
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