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Transformation of Tetrahymena thermophila with a mutated circular ribosomal DNA plasmid vector.
A circular plasmid containing a complete Tetrahymena thermophila rRNA gene (rDNA), with a tandem repeat of a 1.9-kilobase-pair segment encompassing the replication origin and the rRNA promoter, and a polylinker in the 3' nontranscribed spacer, was used to transform T. thermophila by microinject...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1989
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC298307/ https://ncbi.nlm.nih.gov/pubmed/2813408 |
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