Transformation of Tetrahymena thermophila with a mutated circular ribosomal DNA plasmid vector.
A circular plasmid containing a complete Tetrahymena thermophila rRNA gene (rDNA), with a tandem repeat of a 1.9-kilobase-pair segment encompassing the replication origin and the rRNA promoter, and a polylinker in the 3' nontranscribed spacer, was used to transform T. thermophila by microinjection....
Enregistré dans:
| Publié dans: | Proc Natl Acad Sci U S A |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
National Academy of Sciences
1989
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC298307/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2813408/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.21.8487 |
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