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"Activated"-RecA protein affinity chromatography of LexA repressor and other SOS-regulated proteins.

We have developed an affinity column to study the interaction of LexA repressor and other substrates with the activated form of RecA protein. Nucleoprotein complexes of RecA protein, (dT)25-30, and adenosine 5'-[gamma-S]thio-triphosphate were formed in solution and bound to RecA protein-agarose...

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Foilsithe in:Proc Natl Acad Sci U S A
Main Authors: Freitag, N, McEntee, K
Formáid: Artigo
Teanga:Inglês
Foilsithe: National Academy of Sciences 1989
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC298281/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2554312/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.21.8363
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