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Assay of vacuolar pH in yeast and identification of acidification-defective mutants.
As part of a genetic analysis of the biogenesis and function of the vacuole (lysosome) in the yeast Saccharomyces cerevisiae, assays of vacuolar pH were developed and used to identify mutants defective in vacuolar acidification. Vacuoles were labeled with 6-carboxyfluorescein with the membrane-perme...
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| Publicado en: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
National Academy of Sciences
1989
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC297985/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2674942/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.18.7027 |
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