Genetic Transformation and Mutagenesis via Single-Stranded DNA in the Unicellular, Diazotrophic Cyanobacteria of the Genus Cyanothece

We describe a genetic system for producing specific gene knockouts in Cyanothece sp. strain PCC 7822 using a single-stranded DNA technique (B. Zorin, P. Hegemann, and I. Sizova, Eukaryot. Cell 4:1264-1272, 2005). The first fully segregated mutant was a ΔnifK mutant, and it was unable to grow on medi...

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Bibliographic Details
Main Authors: Min, Hongtao, Sherman, Louis A.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 2010
Subjects:
Genetics and Molecular Biology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2976206/
https://ncbi.nlm.nih.gov/pubmed/20851971
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01456-10
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