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Genetic Transformation and Mutagenesis via Single-Stranded DNA in the Unicellular, Diazotrophic Cyanobacteria of the Genus Cyanothece
We describe a genetic system for producing specific gene knockouts in Cyanothece sp. strain PCC 7822 using a single-stranded DNA technique (B. Zorin, P. Hegemann, and I. Sizova, Eukaryot. Cell 4:1264-1272, 2005). The first fully segregated mutant was a ΔnifK mutant, and it was unable to grow on medi...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2976206/ https://ncbi.nlm.nih.gov/pubmed/20851971 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01456-10 |
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