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"Bundle blot" purification and initial protein characterization of hair cell stereocilia.

Stereocilia were isolated from bullfrog (Rana catesbeiana) saccular hair cells by nitrocellulose adhesion. The high purity and high yield of the preparation were demonstrated by microscopy. SDS/PAGE of stereociliary proteins resolved 12-15 major bands. Actin, previously identified as a component of...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Principais autores: Shepherd, G M, Barres, B A, Corey, D P
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1989
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC297538/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2662191/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.13.4973
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