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F-actin affinity chromatography: technique for isolating previously unidentified actin-binding proteins.

We have developed stable and easy to use filamentous actin (F-actin) affinity-chromatography columns that selectively purify proteins that bind to actin filaments from cell extracts. Most traditional assays for actin-associated proteins screen for their effects on actin polymerization or actin filam...

Ausführliche Beschreibung

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Veröffentlicht in:Proc Natl Acad Sci U S A
Hauptverfasser: Miller, K G, Alberts, B M
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 1989
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Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC297504/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2740328/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.13.4808
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