Microfluidic image cytometry for quantitative single-cell profiling of human pluripotent stem cells in chemically defined conditions

Microfluidic image cytometry (MIC) has been developed to study phenotypes of various hPSC lines by screening several chemically defined serum/feeder-free conditions. A chemically defined hPSC culture was established using 20 ng mL(−1) of bFGF on 20 μg mL(−1) of Matrigel to grow hPSCs over a week in...

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Bibliografiska uppgifter
Huvudupphovsmän: Kamei, Ken-ichiro, Ohashi, Minori, Gschweng, Eric, Ho, Quinn, Suh, Jane, Tang, Jinghua, Yu, Zeta Tak For, Clark, Amander T., Pyle, April D., Teitell, Michael A., Lee, Ki-Bum., Witte, Owen N., Tseng, Hsian-Rong
Materialtyp: Artigo
Språk:Inglês
Publicerad: 2010
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Article
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC2970622/
https://ncbi.nlm.nih.gov/pubmed/20390128
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/b922884e
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