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Isolation of segments of homologous genes with only one conserved amino acid region via PCR

We present a method which allows the isolation of fragments from genes coding for homologous proteins via PCR when only one block of conserved amino acids is available. Sets of degenerated primers are defined by reverse translation of the conserved amino acids such that each set contains not more th...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Laging, Martin, Fartmann, Berthold, Kramer, Wilfried
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Oxford University Press 2001
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC29689/
https://ncbi.nlm.nih.gov/pubmed/11139638
Tagiau: Ychwanegu Tag
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