Immobilization and Characterization of a Recombinant Thermostable Lipase (Pf2001) from Pyrococcus furiosus on Supports with Different Degrees of Hydrophobicity

We studied the immobilization of a recombinant thermostable lipase (Pf2001Δ60) from the hyperthermophilic archaeon Pyrococcus furiosus on supports with different degrees of hydrophobicity: butyl Sepabeads and octadecyl Sepabeads. The enzyme was strongly adsorbed in both supports. When it was adsorbe...

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Detalhes bibliográficos
Main Authors: Branco, Roberta Vieira, Estrada Gutarra, Melissa Limoeiro, Freire, Denise Maria Guimarães, Almeida, Rodrigo Volcan
Formato: Artigo
Idioma:Inglês
Publicado em: SAGE-Hindawi Access to Research 2010
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2967836/
https://ncbi.nlm.nih.gov/pubmed/21052496
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4061/2010/180418
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