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Enumeration of islets by nuclei counting and light microscopic analysis

Islet enumeration in impure preparations by conventional dithizone staining and visual counting is inaccurate and operator dependent. We examined nuclei counting for measuring the total number of cells in islet preparations, and we combined it with morphological analysis by light microscopy (LM) for...

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Detalhes bibliográficos
Main Authors: Pisania, Anna, Papas, Klearchos K., Powers, Daryl E., Rappel, Michael J., Omer, Abdulkadir, Bonner-Weir, Susan, Weir, Gordon C., Colton, Clark K.
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2966546/
https://ncbi.nlm.nih.gov/pubmed/20697375
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/labinvest.2010.125
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