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Analysis of conditional gene deletion using probe based Real-Time PCR

BACKGROUND: Conditional gene deletion using Cre-lox recombination is frequently used in mouse genetics; however recombination is frequently incomplete, resulting in a mixture of cells containing the functional (2lox) allele and the truncated (1lox) allele. Conventional analysis of 1lox/2lox allele r...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Weis, Britta, Schmidt, Joachim, Lyko, Frank, Linhart, Heinz G
Format: Artigo
Sprache:Inglês
Veröffentlicht: BioMed Central 2010
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2966447/
https://ncbi.nlm.nih.gov/pubmed/20950424
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-10-75
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