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A novel culture system for mouse spermatid maturation which produces elongating spermatids capable of inducing calcium oscillation during fertilization and embryonic development

PURPOSE: To establish an in vitro culture system for mouse round spermatids that models spermiogenesis and enables the assessment of oocyte activation ability. METHODS: Round spermatids and Sertoli cells were isolated from testicular tissues of B6D2F1 male mice and co-cultured in the presence of tes...

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Main Authors: Hasegawa, Hisataka, Terada, Yukihiro, Ugajin, Tomohisa, Yaegashi, Nobuo, Sato, Kahei
Format: Artigo
Jezik:Inglês
Izdano: Springer US 2010
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2965342/
https://ncbi.nlm.nih.gov/pubmed/20526662
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10815-010-9442-3
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