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Confronting two-pair primer design for enzyme-free SNP genotyping based on a genetic algorithm

BACKGROUND: Polymerase chain reaction with confronting two-pair primers (PCR-CTPP) method produces allele-specific DNA bands of different lengths by adding four designed primers and it achieves the single nucleotide polymorphism (SNP) genotyping by electrophoresis without further steps. It is a time...

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Bibliographic Details
Main Authors: Yang, Cheng-Hong, Cheng, Yu-Huei, Chuang, Li-Yeh, Chang, Hsueh-Wei
Format: Artigo
Language:Inglês
Published: BioMed Central 2010
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2964683/
https://ncbi.nlm.nih.gov/pubmed/20942913
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2105-11-509
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