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A novel multiple affinity purification tag and its use in identification of proteins associated with a cyclin–CDK complex

A novel multiple affinity purification (MAFT) or tandem affinity purification (TAP) tag has been constructed. It consists of the calmodulin binding peptide, six histidine residues, and three copies of the hemagglutinin epitope. This ‘CHH’ MAFT tag allows two or three consecutive purification steps,...

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Detalles Bibliográficos
Main Authors: Honey, Sangeet, Schneider, Brandt L., Schieltz, David M., Yates, John R., Futcher, Bruce
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2001
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC29631/
https://ncbi.nlm.nih.gov/pubmed/11160944
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