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STED nanoscopy in living cells using Fluorogen Activating Proteins

We demonstrate the effectiveness of a genetically encoded Malachite Green (MG) binding fluorogen activating protein (FAP) for live cell stimulated emission depletion nanoscopy (STED). Both extracellular and intracellular FAPs were tested in living cells using fluorogens with either membrane expresse...

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Main Authors: Fitzpatrick, James A.J., Yan, Qi, Sieber, Jochen J., Dyba, Marcus, Schwarz, Ulf, Szent-Gyorgyi, Chris, Woolford, Carol A., Berget, Peter B., Waggoner, Alan S., Bruchez, Marcel P.
格式: Artigo
語言:Inglês
出版: 2009
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC2957894/
https://ncbi.nlm.nih.gov/pubmed/20976031
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bc900249e
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