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Overproduced Brucella abortus PdhS-mCherry forms soluble aggregates in Escherichia coli, partially associating with mobile foci of IbpA-YFP

BACKGROUND: When heterologous recombinant proteins are produced in Escherichia coli, they often precipitate to form insoluble aggregates of unfolded polypeptides called inclusion bodies. These structures are associated with chaperones like IbpA. However, there are reported cases of "non-classic...

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Detalhes bibliográficos
Main Authors: Van der Henst, Charles, Charlier, Caroline, Deghelt, Michaël, Wouters, Johan, Matroule, Jean-Yves, Letesson, Jean-Jacques, De Bolle, Xavier
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2010
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2957392/
https://ncbi.nlm.nih.gov/pubmed/20920169
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2180-10-248
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