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Overproduced Brucella abortus PdhS-mCherry forms soluble aggregates in Escherichia coli, partially associating with mobile foci of IbpA-YFP

BACKGROUND: When heterologous recombinant proteins are produced in Escherichia coli, they often precipitate to form insoluble aggregates of unfolded polypeptides called inclusion bodies. These structures are associated with chaperones like IbpA. However, there are reported cases of "non-classic...

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Autors principals: Van der Henst, Charles, Charlier, Caroline, Deghelt, Michaël, Wouters, Johan, Matroule, Jean-Yves, Letesson, Jean-Jacques, De Bolle, Xavier
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2010
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2957392/
https://ncbi.nlm.nih.gov/pubmed/20920169
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2180-10-248
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