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Overproduced Brucella abortus PdhS-mCherry forms soluble aggregates in Escherichia coli, partially associating with mobile foci of IbpA-YFP

BACKGROUND: When heterologous recombinant proteins are produced in Escherichia coli, they often precipitate to form insoluble aggregates of unfolded polypeptides called inclusion bodies. These structures are associated with chaperones like IbpA. However, there are reported cases of "non-classic...

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Αποθηκεύτηκε σε:
Λεπτομέρειες βιβλιογραφικής εγγραφής
Κύριοι συγγραφείς: Van der Henst, Charles, Charlier, Caroline, Deghelt, Michaël, Wouters, Johan, Matroule, Jean-Yves, Letesson, Jean-Jacques, De Bolle, Xavier
Μορφή: Artigo
Γλώσσα:Inglês
Έκδοση: BioMed Central 2010
Θέματα:
Διαθέσιμο Online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2957392/
https://ncbi.nlm.nih.gov/pubmed/20920169
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2180-10-248
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