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Overproduced Brucella abortus PdhS-mCherry forms soluble aggregates in Escherichia coli, partially associating with mobile foci of IbpA-YFP

BACKGROUND: When heterologous recombinant proteins are produced in Escherichia coli, they often precipitate to form insoluble aggregates of unfolded polypeptides called inclusion bodies. These structures are associated with chaperones like IbpA. However, there are reported cases of "non-classic...

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書誌詳細
主要な著者: Van der Henst, Charles, Charlier, Caroline, Deghelt, Michaël, Wouters, Johan, Matroule, Jean-Yves, Letesson, Jean-Jacques, De Bolle, Xavier
フォーマット: Artigo
言語:Inglês
出版事項: BioMed Central 2010
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC2957392/
https://ncbi.nlm.nih.gov/pubmed/20920169
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2180-10-248
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