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Performance of Nanoliter-Sized Droplet-based Microfluidic PCR

A microfluidic device was used to characterize PCR in aqueous-in-oil droplets for potential point-of-care applications. Droplets with a volume range of 5–250nL can be formed on-chip reproducibly, and PCR in the droplets shows amplification efficiencies comparable to benchtop reactions with no evapor...

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Bibliographic Details
Main Authors: Wang, Fang, Burns, Mark A.
Format: Artigo
Language:Inglês
Published: 2009
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2955802/
https://ncbi.nlm.nih.gov/pubmed/19479169
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10544-009-9324-6
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