Spatial Distribution of the State of Water in Frozen Mammalian Cells

We describe direct determination of the state of intracellular water, measurement of the intercellular concentration of a cryoprotectant agent (dimethylsulfoxide), and the distribution of organic material in frozen mammalian cells. Confocal Raman microspectroscopy was utilized at cryogenic temperatu...

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Detalles Bibliográficos
Main Authors: Dong, Jinping, Malsam, Jason, Bischof, John C., Hubel, Allison, Aksan, Alptekin
Formato: Artigo
Idioma:Inglês
Publicado: The Biophysical Society 2010
Assuntos:
Cellular Biophysics and Electrophysiology
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2955500/
https://ncbi.nlm.nih.gov/pubmed/20959085
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2010.08.035
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