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Structure, function, and folding of phosphoglycerate kinase are strongly perturbed by macromolecular crowding

We combine experiment and computer simulation to show how macromolecular crowding dramatically affects the structure, function, and folding landscape of phosphoglycerate kinase (PGK). Fluorescence labeling shows that compact states of yeast PGK are populated as the amount of crowding agents (Ficoll...

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Main Authors: Dhar, Apratim, Samiotakis, Antonios, Ebbinghaus, Simon, Nienhaus, Lea, Homouz, Dirar, Gruebele, Martin, Cheung, Margaret S.
פורמט: Artigo
שפה:Inglês
יצא לאור: National Academy of Sciences 2010
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גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC2955104/
https://ncbi.nlm.nih.gov/pubmed/20921368
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1006760107
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