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DNA methylation profiling using HpaII tiny fragment enrichment by ligation-mediated PCR (HELP)
The HELP assay is a technique that allows genome-wide analysis of cytosine methylation. Here we describe the assay, its relative strengths and weaknesses, and the transition of the assay from a microarray to massively-parallel sequencing-based foundation.
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| Autores principales: | , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
2010
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2954265/ https://ncbi.nlm.nih.gov/pubmed/20434563 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2010.04.013 |
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