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Preparation of (13)C-labeled ceramide by acetic acid bacteria and its incorporation in mice

We prepared 2-hydroxypalmitoyl-sphinganine (dihydroceramide) labeled with a stable isotope by culturing acetic acid bacteria with (13)C-labeled acetic acid. The GC/MS spectrum of the trimethylsilyl derivative of (13)C-labeled dihydroceramide gave molecular ions with an increased mass of 12–17 Da ove...

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Detalhes bibliográficos
Main Authors: Fukami, Hiroyuki, Tachimoto, Hideki, Kishi, Mikiya, Kaga, Takayuki, Waki, Hatsue, Iwamoto, Machiko, Tanaka, Yasukazu
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Biochemistry and Molecular Biology 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2952581/
https://ncbi.nlm.nih.gov/pubmed/20656918
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D009191
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