Development of pyrF-Based Genetic System for Targeted Gene Deletion in Clostridium thermocellum and Creation of a pta Mutant

We report development of a genetic system for making targeted gene knockouts in Clostridium thermocellum, a thermophilic anaerobic bacterium that rapidly solubilizes cellulose. A toxic uracil analog, 5-fluoroorotic acid (5-FOA), was used to select for deletion of the pyrF gene. The ΔpyrF strain is a...

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Main Authors: Tripathi, Shital A., Olson, Daniel G., Argyros, D. Aaron, Miller, Bethany B., Barrett, Trisha F., Murphy, Daniel M., McCool, Jesse D., Warner, Anne K., Rajgarhia, Vineet B., Lynd, Lee R., Hogsett, David A., Caiazza, Nicky C.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology (ASM) 2010
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Methods
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2950449/
https://ncbi.nlm.nih.gov/pubmed/20693441
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01484-10
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