Development of pyrF-Based Genetic System for Targeted Gene Deletion in Clostridium thermocellum and Creation of a pta Mutant

We report development of a genetic system for making targeted gene knockouts in Clostridium thermocellum, a thermophilic anaerobic bacterium that rapidly solubilizes cellulose. A toxic uracil analog, 5-fluoroorotic acid (5-FOA), was used to select for deletion of the pyrF gene. The ΔpyrF strain is a...

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Détails bibliographiques
Auteurs principaux: Tripathi, Shital A., Olson, Daniel G., Argyros, D. Aaron, Miller, Bethany B., Barrett, Trisha F., Murphy, Daniel M., McCool, Jesse D., Warner, Anne K., Rajgarhia, Vineet B., Lynd, Lee R., Hogsett, David A., Caiazza, Nicky C.
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 2010
Sujets:
Methods
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2950449/
https://ncbi.nlm.nih.gov/pubmed/20693441
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01484-10
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