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Mutation of High-Affinity Methionine Permease Contributes to Selenomethionyl Protein Production in Saccharomyces cerevisiae

The production of selenomethionine (SeMet) derivatives of recombinant proteins allows phase determination by single-wavelength or multiwavelength anomalous dispersion phasing in X-ray crystallography, and this popular approach has permitted the crystal structures of numerous proteins to be determine...

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Autori principali: Kitajima, Toshihiko, Chiba, Yasunori, Jigami, Yoshifumi
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology (ASM) 2010
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2950440/
https://ncbi.nlm.nih.gov/pubmed/20693451
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01026-10
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