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Measuring the effect of ligand binding on the interface stability of multimeric proteins using dynamic light scattering
We have demonstrated that an approach using guanidine hydrochloride at low concentrations to progressively disrupt protein-protein interactions can be quantitated using dynamic light scattering. This approach is sensitive enough to detect ligand-induced changes of subunit–subunit interactions for ho...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2949477/ https://ncbi.nlm.nih.gov/pubmed/20732291 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2010.08.019 |
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