Development of a Novel Fluorescence Assay Based on the Use of the Thrombin-Binding Aptamer for the Detection of O(6)-Alkylguanine-DNA Alkyltransferase Activity

Human O(6)-alkylguanine-DNA alkyltransferase (hAGT) is a DNA repair protein that reverses the effects of alkylating agents by removing DNA adducts from the O(6) position of guanine. Here, we developed a real-time fluorescence hAGT activity assay that is based on the detection of conformational chang...

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Bibliographic Details
Main Authors: Tintoré, Maria, Aviñó, Anna, Ruiz, Federico M., Eritja, Ramón, Fàbrega, Carme
Format: Artigo
Language:Inglês
Published: SAGE-Hindawi Access to Research 2010
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2946612/
https://ncbi.nlm.nih.gov/pubmed/20936180
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4061/2010/632041
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