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Isolation and mapping of a mutation in Escherichia coli with altered levels of ribonuclease H.

A mutant of Escherichia coli with altered levels of ribonuclease (RNase) H was isolated after mutagenesis with ethyl methane sulfonate. A procedure for assaying RNase H in partially purified extracts was used to screen approximately 1,500 colonies for variations in RNase H activity. Confirmation of...

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Bibliographic Details
Main Authors: Carl, P L, Bloom, L, Crouch, R J
Format: Artigo
Language:Inglês
Published: 1980
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC294580/
https://ncbi.nlm.nih.gov/pubmed/6998952
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