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In situ reactivation of glycerol-inactivated coenzyme B12-dependent enzymes, glycerol dehydratase and diol dehydratase.

The catalytic properties of coenzyme B12-dependent glycerol dehydratase and diol dehydratase were studied in situ with Klebsiella pneumoniae cells permeabilized by toluene treatment, since the in situ enzymes approximate the in vivo conditions of the enzymes more closely than enzymes in cell-free ex...

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Bibliographic Details
Main Authors: Honda, S, Toraya, T, Fukui, S
Format: Artigo
Language:Inglês
Published: 1980
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC294534/
https://ncbi.nlm.nih.gov/pubmed/6997273
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