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Molecular Cloning, Expression, and Characterization of a Ca(2+)-Dependent, Membrane-Associated Nuclease of Mycoplasma genitalium

In this study, we identified and characterized the enzymatic properties of MG_186, a calcium-dependent Mycoplasma genitalium nuclease. MG_186 displays the hallmarks of nucleases, as indicated by its amino acid sequence similarity to other nucleases. We cloned, UGA corrected, expressed, purified, and...

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Detalhes bibliográficos
Main Authors: Li, Linbo, Krishnan, Manickam, Baseman, Joel B., Kannan, T. R.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2944508/
https://ncbi.nlm.nih.gov/pubmed/20639320
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.00401-10
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