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Role of nucleases in the isolation of plasmid deoxyribonucleic acid from Pseudomonas cepacia 4G9.

Brij 58-cleared lysates of Pseudomonas cepacia 4G9 contain both exonucleolytic and endonucleolytic activities. Endonuclease activity was unaffected by 125 mM ethylenediaminetetraacetic acid, whereas the exonuclease activity was inhibited. In contrast, Sarkosyl NL97 inhibited only the endonuclease. S...

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Detalles Bibliográficos
Main Authors: Williams, J A, Yeggy, J P, Markovetz, A J
Formato: Artigo
Idioma:Inglês
Publicado: 1980
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC294418/
https://ncbi.nlm.nih.gov/pubmed/6259115
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