Quantitative analysis of conditional gene inactivation using rationally designed, tetracycline-controlled miRNAs

The combination of RNA interference (RNAi) with the tetracycline-controlled transcription activation (tet) system promises to become a powerful method for conditional gene inactivation in cultured cells and in whole organisms. Here, we tested critical sequence elements that originated from miRNA mR-...

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Hlavní autoři: Berger, Stefan M., Pesold, Brigitte, Reber, Simone, Schönig, Kai, Berger, Annette J., Weidenfeld, Ina, Miao, Jun, Berger, Martin R., Gruss, Oliver J., Bartsch, Dusan
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2010
Témata:
Methods Online
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2943624/
https://ncbi.nlm.nih.gov/pubmed/20639530
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq616
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