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Rapid Identification of Bacterial Pathogens in Positive Blood Culture Bottles by Use of a Broad-Based PCR Assay Coupled with High-Resolution Melt Analysis

We evaluated a broad-based PCR assay coupled with high-resolution melt analysis for rapid bacterial identification in patients with bacterial sepsis. With a reference library of 60 clinically relevant bacterial species, 52 positive blood culture samples were tested. Our assay identified 46/52 sample...

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Detalhes bibliográficos
Main Authors: Won, Helen, Rothman, Richard, Ramachandran, Padmini, Hsieh, Yu-Hsiang, Kecojevic, Aleksandar, Carroll, Karen C., Aird, Deborah, Gaydos, Charlotte, Yang, Samuel
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2937718/
https://ncbi.nlm.nih.gov/pubmed/20631110
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00718-10
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