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Analysis of protein kinase specificity using arrayed positional scanning peptide libraries

Protein kinases vary substantially in their consensus phosphorylation motifs, the residues that are either preferred or deselected by the kinase at specific positions surrounding the phosphorylation site. The protocol described here is used to rapidly determine phosphorylation motifs for serine-thre...

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Hlavní autoři: Chen, Catherine, Turk, Benjamin E.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2010
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2937348/
https://ncbi.nlm.nih.gov/pubmed/20583094
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471142727.mb1814s91
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