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Isolation and Characterization of a Composite Plasmid Rms201 Mutant Temperature Sensitive for Replication

A mutant temperature-sensitive for R-plasmid replication, Rms201ts14, was isolated from composite plasmid Rms201 after mutagenesis of P1 transducing lysate with 100 mM hydroxylamine for 40 h at 37°C. When Escherichia coli ML1410(Rms201ts14)(+) was grown at temperatures between 40 and 42°C in L broth...

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Detalhes bibliográficos
Main Authors: Ike, Yasuyoshi, Hashimoto, Hajime, Motohashi, Kazuo, Fujisawa, Noriko, Mitsuhashi, Susumu
Formato: Artigo
Idioma:Inglês
Publicado em: 1980
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC293662/
https://ncbi.nlm.nih.gov/pubmed/6988387
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