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Transposition of Tn904 encoding streptomycin resistance into the octopine Ti plasmid of Agrobacterium tumefaciens.

A transfer-deficient derivative of plasmid RP1-pMG1 was isolated after insertion of Mu cts62. The Tra- R plasmid was used to donate Tn904, encoding streptomycin resistance, to Ti plasmid pAL102 harbored by Agrobacterium tumefaciens Ach5. Under conditions promoting high Ti transfer frequencies, 155 s...

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Main Authors: Klapwijk, P M, van Breukelen, J, Korevaar, K, Ooms, G, Schilperoort, R A
格式: Artigo
語言:Inglês
出版: 1980
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC293545/
https://ncbi.nlm.nih.gov/pubmed/7353997
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