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Cloning, expression, purification, crystallization and preliminary X-ray diffraction studies of the catalytic domain of a hyperthermostable endo-1,4-β-d-mannanase from Thermotoga petrophila RKU-1

Endo-1,4-β-d-mannanases play key roles in seed germination and fruit ripening and have recently received much attention owing to their potential applications in the food, detergent and kraft pulp industries. In order to delineate their structural determinants for specificity and stability, X-ray cry...

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Detalhes bibliográficos
Main Authors: Santos, Camila Ramos, Squina, Fabio Marcio, Navarro, Andreia Meza, Ruller, Roberto, Prade, Rolf, Murakami, Mario Tyago
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2935232/
https://ncbi.nlm.nih.gov/pubmed/20823531
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110029131
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