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New strategy for the synthesis of chemically modified RNA constructs exemplified by hairpin and hammerhead ribozymes
The CuAAC reaction (click chemistry) has been used in conjunction with solid-phase synthesis to produce catalytically active hairpin ribozymes around 100 nucleotides in length. Cross-strand ligation through neighboring nucleobases was successful in covalently linking presynthesized RNA strands with...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2932611/ https://ncbi.nlm.nih.gov/pubmed/20713730 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1006447107 |
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