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The design and validation of a novel phenotypic assay to determine HIV-1 coreceptor usage of clinical isolates

A phenotypic assay to determine coreceptor usage of HIV-1 has been developed for rapid testing of clinical samples. The assay is based on the synthesis of viral stock from full-length env amplicons isolated from patient’s plasma. Pseudoviral stock is generated rapidly by using an overlapping PCR met...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Lin, Nina H., Negusse, Daniel M., Beroukhim, Rameen, Giguel, Francoise, Lockman, Shahin, Essex, Myron, Kuritzkes, Daniel R.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2010
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2930119/
https://ncbi.nlm.nih.gov/pubmed/20599562
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2010.06.012
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