Detection of Early and Single Infections of Schistosoma japonicum in the Intermediate Host Snail, Oncomelania hupensis, by PCR and Loop-Mediated Isothermal Amplification (LAMP) Assay

Polymerase chain reaction (PCR) with the specific primer set amplifying 28S ribosomal DNA (rDNA) of Schistosoma japonicum was able to detect genomic DNA of S. japonicum, but not S. mansoni, at 100 fg. This procedure enabled us to detect the DNA from a single miracidium and a snail infected with one...

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Bibliografiske detaljer
Main Authors: Kumagai, Takashi, Furushima-Shimogawara, Rieko, Ohmae, Hiroshi, Wang, Tian-Ping, Lu, Shaohong, Chen, Rui, Wen, Liyong, Ohta, Nobuo
Format: Artigo
Sprog:Inglês
Udgivet: The American Society of Tropical Medicine and Hygiene 2010
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Articles
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2929049/
https://ncbi.nlm.nih.gov/pubmed/20810818
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4269/ajtmh.2010.10-0016
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