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Quantitative analysis of protein-RNA interactions by gel mobility shift

The gel mobility shift assay is routinely used to visualize protein-RNA interactions. Its power resides in the ability to resolve free from bound RNA with high resolution in a gel matrix. In this chapter, we review the quantitative application of this approach to elucidate thermodynamic properties o...

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Detalhes bibliográficos
Main Authors: Ryder, Sean P., Recht, Michael I., Williamson, James R.
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2928675/
https://ncbi.nlm.nih.gov/pubmed/18982286
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-60327-475-3_7
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