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Quantitative analysis of protein-RNA interactions by gel mobility shift
The gel mobility shift assay is routinely used to visualize protein-RNA interactions. Its power resides in the ability to resolve free from bound RNA with high resolution in a gel matrix. In this chapter, we review the quantitative application of this approach to elucidate thermodynamic properties o...
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| Главные авторы: | , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2008
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2928675/ https://ncbi.nlm.nih.gov/pubmed/18982286 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-60327-475-3_7 |
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