Establishment of a minor groove binder-probe based quantitative real time PCR to detect Borrelia burgdorferi sensu lato and differentiation of Borrelia spielmanii by ospA-specific conventional PCR

BACKGROUND: Borrelia burgdorferi sensu lato (sl), the causative agent of Lyme borreliosis, is transmitted by ticks of the genus Ixodes as vector. For identification of Borrelia infections in ticks a TaqMan™ minor groove binder (MGB) probe-based quantitative real time PCR (qPCR) was established targe...

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Main Authors: Strube, Christina, Montenegro, Victor M, Epe, Christian, Eckelt, Elke, Schnieder, Thomas
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2010
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2928209/
https://ncbi.nlm.nih.gov/pubmed/20698952
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-3305-3-69
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