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Knocking down gene function with an RNA aptamer expressed as part of an intron
We developed a powerful expression system to produce aptamers and other types of functional RNA in yeast to examine their effects. Utilizing the intron homing process, the aptamer-coding sequences were integrated into hundreds of rRNA genes, and the aptamers were transcribed at high levels by RNA po...
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| Huvudupphovsmän: | , , , , , |
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| Materialtyp: | Artigo |
| Språk: | Inglês |
| Publicerad: |
Oxford University Press
2010
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| Ämnen: | |
| Länkar: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2926621/ https://ncbi.nlm.nih.gov/pubmed/20542918 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq529 |
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