The ROCK Inhibitor Y-27632 Improves Recovery of Human Embryonic Stem Cells after Fluorescence-Activated Cell Sorting with Multiple Cell Surface Markers

BACKGROUND: Due to the inherent sensitivity of human embryonic stem cells (hESCs) to manipulations, the recovery and survival of hESCs after fluorescence-activated cell sorting (FACS) can be low. Additionally, a well characterized and robust methodology for performing FACS on hESCs using multiple-ce...

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Autors principals: Emre, Nil, Vidal, Jason G., Elia, Jeanne, O'Connor, Eric D., Paramban, Rosanto I., Hefferan, Michael P., Navarro, Roman, Goldberg, Danielle S., Varki, Nissi M., Marsala, Martin, Carson, Christian T.
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2010
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2921395/
https://ncbi.nlm.nih.gov/pubmed/20730054
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0012148
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