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Expression and characterization of the FXYD ion transport regulators for NMR structural studies in lipid micelles and lipid bilayers

The proteins PLM (phospholemman), CHIF (channel inducing factor), and Mat8 (mammary tumor protein 8 kDa) are members of the FXYD family of ion transport regulatory membrane proteins. Here we describe their cloning and expression in Escherichia coli, and their purification for NMR structural studies...

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Detalhes bibliográficos
Main Authors: Crowell, Kevin J., Franzin, Carla M., Koltay, Anita, Lee, Sangmin, Lucchese, Anna Maria, Snyder, Bradley C., Marassi, Francesca M.
Formato: Artigo
Idioma:Inglês
Publicado em: 2003
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2917601/
https://ncbi.nlm.nih.gov/pubmed/12535606
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