Overexpression, crystallization and preliminary X-­ray analysis of xylulose-5-phosphate/fructose-6-phosphate phosphoketolase from Bifidobacterium breve

The xylulose-5-phosphate/fructose-6-phosphate phosphoketolase gene from Bifidobacterium breve was cloned and overexpressed in Escherichia coli. The enzyme was purified to homogeneity and crystallized by the sitting-drop vapour-diffusion method. Crystals were obtained at 293 K using 0.05 mM thiamine...

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Bibliografiske detaljer
Main Authors: Suzuki, Ryuichiro, Kim, Byung-Jun, Shibata, Tsuyoshi, Iwamoto, Yuki, Katayama, Takane, Ashida, Hisashi, Wakagi, Takayoshi, Shoun, Hirofumi, Fushinobu, Shinya, Yamamoto, Kenji
Format: Artigo
Sprog:Inglês
Udgivet: International Union of Crystallography 2010
Fag:
Crystallization Communications
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2917298/
https://ncbi.nlm.nih.gov/pubmed/20693675
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110023845
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