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Hydroxyapatite-Mediated Separation of Double-Stranded DNA, Single-Stranded DNA, and RNA Genomes from Natural Viral Assemblages

Metagenomics can be used to determine the diversity of complex, often unculturable, viral communities with various nucleic acid compositions. Here, we report the use of hydroxyapatite chromatography to efficiently fractionate double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), dsRNA, and ssRNA...

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Bibliografiske detaljer
Main Authors: Andrews-Pfannkoch, Cynthia, Fadrosh, Douglas W., Thorpe, Joyce, Williamson, Shannon J.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2010
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2916501/
https://ncbi.nlm.nih.gov/pubmed/20543058
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00204-10
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