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High-Resolution Melt Curve Analysis for Identification of Single Nucleotide Mutations in the Quinolone Resistance Gene aac(6′)-Ib-cr
We have developed a simple PCR-based high-resolution melt curve analysis for identification of the quinolone resistance gene aac(6′)-Ib-cr through regions encompassing the two defining single nucleotide mutations. Dissociation curves showed 100% concordance with DNA sequencing, including the identif...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2916293/ https://ncbi.nlm.nih.gov/pubmed/20498320 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.00485-10 |
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