Optimus Primer: A PCR enrichment primer design program for next-generation sequencing of human exonic regions

BACKGROUND: Polymerase chain reaction (PCR) remains a simple, flexible, and inexpensive method for enriching genomic regions of interest for next-generation sequencing. In order to utilize PCR in this context, a major challenge facing researchers is how to generate a very large number of functional...

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Autors principals: Brown, Andrew MK, Lo, Ken Sin, Guelpa, Paul, Beaudoin, Mélissa, Rioux, John D, Tardif, Jean-Claude, Phillips, Michael S, Lettre, Guillaume
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2010
Matèries:
Technical Note
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2916007/
https://ncbi.nlm.nih.gov/pubmed/20609249
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-3-185
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